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Cell Culture Academy

Articles and insights on cell culture techniques and practices, shared to support learning, discussion, and continuous improvement in the lab.

Key Considerations for Culturing NK-92 and and NK-92MI Cells

NK-92 and NK-92MI share many similarities, but their culture methods and handling precautions differ in some aspects. Here, Pricella provides a detailed explanation of the key considerations for culturing NK-92 cells and NK-92MI cells.

Learn More >> Jan 03,2025

Does BEAS-2B Require Serum for Culture?

BEAS-2B, an SV40-immortalized human bronchial epithelial cell line, is used in toxicology and respiratory research. Serum-free culture preserves epithelial morphology, while serum induces squamous differentiation and boosts metabolic activity.

Learn More >> Jan 02,2025

How to Handle Floating SK-BR-3 Cells?

Learn how to manage floating SK-BR-3 cells, which commonly occur after cryopreservation or passaging.

Learn More >> Jan 02,2025

How to Use PCR for Cell Species Identification

This paper introduces the most commonly used cell species identification method, using pcr method for cell species identification.

Learn More >> Jan 02,2025

A Deep Dive into the HEK293 Cell Line Family

There are several derived sublines of hek293 cells, each with distinct characteristics and applications. Pricella introduces the key features and uses of four major hek293 subtypes: 293T, 293F, 293H, and 293S.

Learn More >> Jan 02,2025

Comprehensive Overview of Astrocyte Knowledge - Pricella

Pricella provides a summary of astrocyte classification, identification, primary isolation, and culture methods, along with key precautions to help you get started quickly with astrocyte-related experiments.

Learn More >> Jan 02,2025

Essential Techniques for Accurate Cell Counting in the Lab - Pricella

Pricella compiled some commonly used counting methods: manual cell counting, automated cell counters, and microscopy-based density estimations for both suspension and adherent cells at varying densities to help you quickly get started with your experiments.

Learn More >> Jan 02,2025

Classification and Selection Criteria for Cytokine Products

This article is about classification and selection criteria for cytokine products.

Learn More >> Jan 02,2025

How to Optimize the Culturing of H9c2 (2-1) Cells

H9c2 cells derived from embryonic BD1X rat heart tissue, you can learn more about the key points to consider when culturing H9c2 (2-1) cells.

Learn More >> Jan 02,2025

Culturing Bone Marrow-Derived Dendritic Cells (DCs)

Dendritic cells (DCs) are named for their pseudopod-like or dendrite-like projections. They are the most versatile and specialized antigen-presenting cells (APCs) in the body, with a unique ability to activate naïve T cells and initiate primary immune responses.DCs are present in extremely low...

Learn More >> Dec 31,2024

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