ManualPM153110
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Product Introduction
Background
Advanced RPMI-1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI-1640, serum supplementation can be reduced by 50–90% with no change in cellular prolife ratio or morphology. Many cell lines do not need to be domesticated to use this medium. Cells successfully cultured in Advanced RPMI-1640, with no adaptation, include SP2/0, Vero, Raji, Daudi, Jurkat, Clone E6-1, THP-1, 4T1, CT26.WT, PC-12 (High differentiation), 22RV1, BxPC-3, B16-F10, etc.
Advanced RPMI-1640 Advanced MEM medium can achieve the serum-reduced effect due to the addition of the following ingredients: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin and various trace elements (sodium selenite, ammonium metavanadate, copper sulfate and manganese chloride).
Ingredient Statement
With
• 2000 mg/L D-Glucose
• 300 mg/L L-Glutamine
• 110 mg/L Sodium Pyruvate
• 5 mg/L Phenol red
• NEAA
• Purified Water Dissolvent
Without
• HEPES
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. Not all cells are suitable for reduced serum culture. Be sure to test the effect in a small amount before replacing it. If necessary, the FBS concentration should be optimized for each cell line to obtain maximum serum reduction.
3. This product has been filtered and sterilized. Pay attention to aseptic operation to avoid contamination.
4. To maintain the best use of this product, do not perform freeze-thaw treatment.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Advanced RPMI-1640 complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.0-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 12 months |
| Storage Condition | This product can be stored at 2-8°C for 12 months with shading light. |
| Shipping Condition | Ice bag |
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FAQs
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Q:1 I am currently culturing cells in a medium supplemented with 10% fetal bovine serum (FBS), and the cells are in excellent condition. What are the advantages of switching to Advanced Medium?
AnswerAdvanced media can reduce serum usage by 50-90%. For most cell lines, a 50% dosage reduction can be achieved without additional adaptation procedures. The advantages of reducing serum usage include the following: 1. It cuts costs, especially during periods of rising serum prices. 2. It extends the service life of each serum batch, thereby reducing experimental variability.
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Q:2 If the basic culture medium is frozen at -20℃, will it affect its use?
AnswerIf the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
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Q:3 How long can I keep my culture media after supplementing with serum?
AnswerGenerally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
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Q:4 My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
AnswerWe routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
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Q:5 How light sensitive is media? Should I also be protecting it from LED light?
AnswerWhile we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
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Q:6 I see a precipitate in my medium, and a change in the pH. What should I do?
AnswerThis is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
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Q:7 Where can I find the osmolality for Medium?
AnswerThe osmolality is listed in the COA for the particular lot number of the medium.
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Q:8 Do you provide customized culture media services?
AnswerYes. We can customize culture media with reduced components or changes in the concentration of existing components.
