Serum-Free Media
Serum-free media (SFM) are a type of cell culture medium formulated without animal serum, designed to support the growth and maintenance of cells while minimizing variability and contamination risks. Our R&D team has developed SFM specifically for HEK293, CHO, hybridoma, Vero, and human mesenchymal stem cells (hMSCs), completely free from animal-derived components. These ready-to-use media ensure good cell growth, high protein expression, minimal batch variation, and stability. Produced under GMP standards and ISO 13485:2016 certified, our SFM guarantee full traceability and reliability for diverse cell culture applications.
View All Serum-Free MediaKey Features
Provides defined culture conditions
Improves the stability and experimental results
Increases cell growth or productivity
Avoids the influence of serum
Simplifies purification and downstream processing
Procell HEK293 Serum-Free Media are designed to support high-density growth of HEK293 cells, enabling efficient transfection and protein expression, making it ideal for applications in recombinant protein production, antibody development, vaccine manufacturing, and related fields.
| Product Name | Cat. No. | Size |
|---|---|---|
| HEK293 Serum-Free Basal Medium | SF1001 | 500 mL |
| HEK293 Serum-Free Feed A | SF1002 | 50 mL |
| HEK293 Serum-Free Feed B | SF1003 | 5 mL |
CHO Serum-Free Basal Medium is fully chemically defined, free of hydrolysates, proteins, growth factors, and any animal-derived components. It is suitable for high-density suspension cultures of various Chinese Hamster Ovary (CHO) cell subtypes, including CHO-K1, CHO DG44, and CHO-S cells, enabling high-level expression of recombinant proteins and antibodies. When combined with the CHO supplements, Procell CHO Serum-Free Feed A and B, it supports high-density cell growth and viability, achieving even higher levels of protein/antibody expression and quality.
| Product Name | Cat. No. | Size |
|---|---|---|
| CHO Serum-Free Basal Medium | SF2001 | 500 mL |
| CHO Serum-Free Feed A | SF2002 | 100 mL |
| CHO Serum-Free Feed B | SF2003 | 10 mL |
During the process of cell passage, the growth rate of cells cultured in Procell CHO Serum-Free Basal Medium (Cat. No.: SF2001) is comparable to that of products from other companies.
Procell Hybridoma Serum-Free Medium is serum-free and contains low concentrations of protein, specifically developed for high-density growth of hybridoma cells. It is suitable for high-density suspension cultures of various hybridoma cells and for protein and antibody expression.
| Product Name | Cat. No. | Size |
|---|---|---|
| Hybridoma Serum-Free Medium | SF3001 | 500 mL |
After a short-term adaptation period using Procell Hybridoma Serum-Free Medium (Cat. No.: SF3001), hybridoma cells maintain stable proliferation and viability.
Hybridoma cells cultured in batch mode show relatively better growth when using Procell Hybridoma Serum-Free Medium (Cat. No.: SF3001) compared to products from other companies.
In different antibody expression projects, Procell Hybridoma Serum-Free Medium (Cat. No.: SF3001) have shown to achieve higher and more stable expression levels compared to products from other companies.
Procell hMSCs Serum-Free Medium is specifically formulated for the growth and expansion of human mesenchymal stem cells (hMSCs). Compared to traditional serum-supplemented media (DMEM + 10% FBS), Procell hMSCs Serum-Free Medium better promotes hMSCs growth and enhances consistency. Using this SFM, hMSCs can be expanded for over five passages while retaining their mesodermal differentiation potential across three lineages- osteocytes, chondrocytes, and adipocytes. Each batch of hMSCs Serum-Free Medium is validated using hMSCs to ensure consistent performance, and hMSCs cultured in serum-supplemented media can transition directly to Procell hMSCs Serum-Free Medium with little to no adaptation required. Additionally, this SFM can also be used for the direct expansion of hMSCs from primary human bone marrow.
| Product Name | Cat. No. | Size |
|---|---|---|
| Human Mesenchymal Stem Cells (hMSCs) Serum-Free Medium | CM-SC01 | 500 mL |
| Human Mesenchymal Stem Cells Serum-Free Medium, with Serum Replacement | CM-SC02 | 500 mL |
hMSCs were cultured and expanded using Procell hMSCs Serum-Free Medium (Cat. No.: CM-SC01). Flow cytometry was used to assess the expression of cell surface antigens. The results showed negative expression for CD19 (0.35%), CD34 (1.23%), and CD45 (1.08%), while positive expression was observed for CD90 (99.94%) and CD105 (97.02%).
Serum-Free Adaptation Process
Step 1: Seed cells in the original medium (serum-supplemented medium) and, once they enter the exponential growth phase, initiate serum-free adaptation.
Step 2: Culture cells in a medium composed of 75% original medium and 25% SFM.
Step 3: Culture cells in a medium composed of 50% original medium and 50% SFM.
Step 4: Culture cells in a medium composed of 25% original medium and 75% SFM.
Step 5: Culture cells in 100% SFM.
Step 1: Seed cells in the original medium (serum-supplemented medium) and, once they enter the exponential growth phase, initiate serum-free adaptation.
Step 2: Culture cells in a medium composed of 75% original medium and 25% SFM.
Step 3: Culture cells in a medium composed of 50% original medium and 50% SFM.
Step 4: Culture cells in a medium composed of 25% original medium and 75% SFM.
Step 5: Culture cells in 100% SFM.
Tips: If cells cannot adapt smoothly from 75% to 100% SFM, consider adding a step with 10% original medium and 90% SFM. For each step, it is recommended to maintain the respective ratios and passage cells for 2-3 generations. If adaptation is successful, the number of passages at each step may be reduced based on actual conditions.
