Cell transfection introduces exogenous nucleic acids into cells to enable intracellular expression or function and is widely used in gene function studies, disease modeling, and gene therapy.

However, variations in membrane structure and endocytic capacity across cell types often prevent general-purpose transfection reagents from achieving both high efficiency and strong cell viability.

Based on the transfection characteristics of different cell types, Procell has developed a series of cell-specific transfection reagents that provide efficient nucleic acid delivery with low toxicity and high reproducibility.

View All Transfection Reagents

Key Features

High Transfection Efficiency

Optimized for individual cell lines, delivering higher transfection success and enhanced protein expression.

Low Cell Toxicity

A low-toxicity formulation that preserves cell viability and ensures reliable downstream functional assays.

Ease of Use

No medium change or complex removal steps required. Just add and incubate-two steps ensure rapid, stress-free transfection.

Technical Support

Procell's technical support team provides real-time response to help you obtain optimal results with confidence.

Product Portfolio

Select the appropriate DNA-, mRNA-, or siRNA-specific transfection reagent for each cell type to achieve plasmid expression, mRNA expression, and gene silencing, with ready-to-use convenience.

DNA Transfection Reagents

CHO-K1	COS-7	Hela	Hep G2
MCF7	NIH/3T3	Vero

mRNA Transfection Reagents

A549	CHO-K1	COS-7	Hela
Hep G2	MCF7	NIH/3T3	THP-1
RAW 264.7	Vero

siRNA Transfection Reagents

A549	Hela	Hep G2	MCF7
NIH/3T3	RAW 264.7	THP-1

Transfection Performance

· DNA Transfection

Procell Mergene1000^® Hep G2 Cell-Specific DNA Transfection Reagent outperforms T Brand L3000 in EGFP-DNA delivery to Hep G2 cells.

	Bright-Field Microscopy	Immunofluorescence (EGFP)	Flow Cytometry
Mergene1000^®
T Brand L3000

Across multiple cell lines, cell-matched Procell Mergene1000^® Cell-Specific DNA Transfection Reagent outperforms T Brand L3000 in EGFP-DNA delivery.

· mRNA Transfection

Procell Mergene1000^® CHO-K1 Cell-Specific mRNA Transfection Reagent outperforms T Brand L3000 in EGFP-mRNA delivery to CHO-K1 cells.

	Bright-Field Microscopy	Immunofluorescence (EGFP)	Flow Cytometry
Mergene1000^®
T Brand L3000

Across multiple cell lines, cell-matched Procell Mergene1000^® Cell-Specific mRNA Transfection Reagent outperforms T Brand L3000 in EGFP-mRNA delivery.

· siRNA Transfection

Procell Mergene1000^® Hela Cell-Specific siRNA Transfection Reagent outperforms T Brand L3000 in FAM-siRNA delivery to Hela cells.

	Bright-Field Microscopy	Fluorescence (FAM)
Mergene1000^®
T Brand L3000

Across multiple cell lines, cell-matched Procell Mergene1000^® Cell-Specific siRNA Transfection Reagent outperforms T Brand L3000 in GAPDH-siRNA silencing efficiency.

FAQs

Can antibiotics be added to the culture medium during transfection?
Answer
Yes. Mergene1000^® transfection reagents can be used with or without antibiotics without significantly affecting experimental results. However, antibiotic-free conditions are recommended for cells that are highly sensitive to transfection or prone to cytotoxicity.
Does passage number affect transfection efficiency?
Answer
Yes. Higher passage numbers may cause genomic and phenotypic changes that reduce transfection efficiency. Use low-passage, healthy cells for optimal results.
What causes high cytotoxicity during transfection?
Answer
Common causes include excessive transfection reagent, prolonged incubation of transfection complexes, poor cell condition, or antibiotics in the culture medium. Follow the conditions recommended in the product protocol.
What causes precipitation in transfection complexes?
Answer
Precipitation usually results from excessive transfection reagent or nucleic acids, or insufficient reaction volume, leading to charge aggregation. Prepare the transfection mixture according to the recommended ratios in the protocol.
What causes inconsistent transfection results?
Answer

This may be related to reagent stability, nucleic acid quality, or cell condition.

Store transfection reagents as recommended in the protocol and avoid repeated freeze-thaw cycles.

Store nucleic acids according to usage frequency: keep at 4℃ for short-term use; if not used for more than 10 days, store at −20℃ or −80℃.

Maintain consistent cell density, growth conditions, and handling procedures to ensure stable and reproducible results.