Cell dissociation reagents enable efficient cell detachment by degrading cell-cell and cell-matrix adhesion molecules, leading to loss of adhesion and cell rounding.

The Procell Cell Dissociation Reagents comprise animal-derived enzymes, recombinant enzymes, enzyme blends, and chemical dissociation systems, supporting diverse applications from routine cell to stem cell dissociation.

Selecting the Appropriate Cell Dissociation Reagent

	Trypsin	Recombinant Trypsin	Accutase
Source	Animal-derived (porcine)	Animal origin–free	Animal origin–free
Buffer	PBS / D-Hank’s	DPBS	DPBS (Ca²⁺- and Mg²⁺-free)
Activity	Concentration-dependent dissociation strength	Gentle, effective	Gentler, high efficiency
Inactivation	Serum or trypsin inhibitor solution	Inactivation by dilution	No inactivation required
Higher cell viability		√	√

Note: For applications involving stem cells, primary cells, or surface marker analysis, gentle dissociation systems are preferred.

Product Portfolio

Trypsin is one of the most commonly used enzymes for dissociating adherent cells and tissues. Procell Trypsin Solution maintains consistent, batch-to-batch performance.

Product Name	Cat. No.	Size	Storage Condition
0.05% Trypsin Solution, dissolved in PBS	PB180218	100 mL	-5--20℃
0.05% Trypsin Solution, with EDTA, dissolved in PBS	PB180219	100 mL	-5--20℃
0.05% Trypsin Solution, dissolved in D-Hank's	PB180220	100 mL	-5--20℃
0.05% Trypsin Solution, with EDTA, dissolved in D-Hank's	PB180221	100 mL	-5--20℃
0.05% Trypsin Solution, with EDTA, phenol red, dissolved in D-Hank's	PB180222	100 mL	-5--20℃
0.25% Trypsin Solution, with phenol red, dissolved in D-Hank's	PB180224	100 mL	-5--20℃
0.25% Trypsin Solution, with EDTA, dissolved in PBS	PB180225	125 mL/500 mL	-5--20℃
0.25% Trypsin Solution, with EDTA, phenol red, dissolved in D-Hank's	PB180226	125 mL/500 mL	-5--20℃
0.25% Trypsin Solution, with EDTA, dissolved in D-Hank's	PB180227	100 mL	-5--20℃
0.25% Trypsin Solution, dissolved in PBS	PB180228	100 mL	-5--20℃
0.25% Trypsin Solution, with EDTA, phenol red, dissolved in PBS	PB180229	100 mL	-5--20℃

Recombinant Trypsin Solution is animal origin–free, provides mild dissociation, and effectively replaces conventional animal-derived trypsin.

Product Name	Cat. No.	Size	Storage Condition
Recombinant Trypsin Solution, without phenol red	PB180240	100 mL/ 500 mL	2-8℃
Recombinant Trypsin Solution, with phenol red	PB180241	100 mL/ 500 mL	2-8℃

Accutase Cell Detachment Solution is a multi-enzyme dissociation solution with proteolytic and collagenolytic activities, suitable for cells sensitive to dissociation conditions.

Gentle Dissociation

Efficient Separation

Preservation of Cell Viability

Protection of Surface Antigens

Product Name	Cat. No.	Size	Storage Condition
Accutase Cell Detachment Solution	PB180201	100 mL	-5--20℃

EDTA chelates divalent cations (e.g., Ca²⁺ and Mg²⁺), disrupting calcium-dependent adhesion. It is applicable for preserving cell surface molecules and commonly used with Trypsin Solution at defined ratios.

Product Name	Cat. No.	Size	Storage Condition
0.5% EDTA Solution	PB180620	100 mL	2-8℃
0.02% EDTA Solution (Versene Solution)	PB180320	100 mL	2-8℃

Validation of Cell Dissociation Reagents

· 0.25% Trypsin Solution

0.25% Trypsin Solution, with EDTA in PBS (Cat. No.: PB180225) was used to dissociate 293T and NCI-H358 cells.Digestion for 2 min in 293T cells and 5 min in NCI-H358 cells enabled uniform detachment with intact morphology. After reseeding and 24 h of culture, cells showed normal morphology and good adherence.

Cell name	Before digestion	After digestion	Incubate for 24 h after digestion
293T
NCI-H358

Note: Digestion time may vary depending on cell condition; the times provided above are for reference only.

· Recombinant Trypsin Solution

Recombinant Trypsin Solution, with phenol red (Cat. No.: PB180241) was used to dissociate Rat Umbilical Cord Mesenchymal Stem Cells. Digestion was completed rapidly, and extended incubation had no significant effect on cell viability or morphology.

Before digestion	After digestionfor 1-3 min	Incubate for 48 h after digestion for 1-3 min
Before digestion	After digestion for 30 min	Incubate for 48 h after digestion for 30 min

· Accutase Cell Detachment Solution

Accutase Cell Detachment Solution (Cat. No.: PB180201) was used to dissociate Rat Bone Marrow Mesenchymal Stem Cells. After 1 min, cells detached uniformly with preserved morphology. Cells were then seeded into 6-well plates at a 1:3 ratio. By day 3, cultures reached full confluence, yielding > 3×10⁶ cells. Flow cytometry confirmed >95% positivity for CD44 and CD90.

Before digestion	After digestion

Flow cytometry（CD44+ 99.99%；CD90+ 96.78%）

FAQs

How should Trypsin Solution (with EDTA) be stored?
Answer
Store at -5℃ to -20℃. Aliquot into small volumes, use immediately after thawing, and avoid repeated freeze-thaw cycles.
How long can thawed Trypsin Solution be stored?
Answer
Thawed Trypsin Solution can be stored at 2-8℃ for up to approximately 1 week. Enzymatic activity gradually declines under non-frozen conditions and may be further affected by repeated handling. Variations in cell adhesion strength may also impact dissociation efficiency. For consistent results, aliquot and minimize repeated use.
Can Recombinant Trypsin Solution digestion be stopped using serum-free or serum substitute media?
Answer
Yes. Recombinant Trypsin Solution does not require inhibitors and can be inactivated by dilution. Serum-free media can be used, though at higher cost. Typically, dilution with a cell buffer or basal medium is recommended.
Does Accutase Cell Detachment Solution require inactivation after dissociation?
Answer
No. Accutase Cell Detachment Solution does not require additional washing or inactivation steps.