PB180620
$ 30.00
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Product Introduction
Background
EDTA is a chemical chelating agent that can chelate divalent metal ions such as Ca2+ and Mg2+. Its solution is also called Versene solution. Because Ca2+ and Mg2+ are important components of cell membranes and have the function of agglutinating cells, EDTA can destroy the connection between cells and promote the dissociation of cells into single cells. EDTA is less toxic, cheap, convenient to use, and does not damage cell surface molecules. It only binds to Ca2+ and Mg2+, so it is suitable for cell digestion where cell surface molecules need to be detected. It is also often mixed with trypsin solution in a certain proportion.
Ingredient Statement
With
• 5000 mg/L EDTA·2Na
Without
• HEPES
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
4. EDTA will not be neutralized, and the digested cells should be washed with PBS, otherwise the cells will be hard to adherent.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's 0.5% EDTA Solution complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 0.5% |
| Volume | 100mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 18 months |
| Storage Condition | This product can be stored at 2-8℃ for 18 months with shading light. |
| Shipping Condition | Ice bag |
Publications
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 5.6
Product Cited: 0.5% EDTA Solution
FAQs
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Q:1 How long can I keep trypsin in the refrigerator after thawing?
AnswerGenerally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.
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Q:2 What are the storage conditions for the Trypsin-EDTA (0.25%)?
AnswerWe recommend storing the Trypsin-EDTA (0.25%) at -5°C to -20°C as specified in the Storage section of our product page for this product. Although it is possible to store the trypsin for up to one week at +4°C, we would recommend preparing aliquots and storing them at -5°C to -20°C. These aliquots should be used up after thawing and freeze/thaw cycles should be avoided.
