Exosome Isolation Kit

Exosomes are a class of extracellular vesicles (EVs) with diameters of approximately 30-150 nm. They are secreted by various cell types under both physiological and pathological conditions and play a pivotal role in intercellular communication. Exosomes have demonstrated significant application potential across a wide range of research fields, including tumor microenvironment regulation, immune modulation, and neurodegenerative diseases.

Ultracentrifugation is the classical method for exosome isolation; however, its heavy reliance on specialized equipment and its time-consuming, labor-intensive procedures greatly limit research efficiency and accessibility. To address these challenges, the Procell R&D team has developed a series of Exosome Isolation Kits based on optimized separation strategies. These kits are compatible with different sample types and research needs, providing efficient and convenient solutions for exosome isolation.

View All Exosome Isolation Kit

Key Features

High Purity

Free from cell debris, proteins, and other vesicle contaminants

Superior Stability

Preserve natural membrane structure and properties

Optimal Yield

Achieve efficient retrieval of exosomes

Wide Applicability

Suitable for serum, plasma, cell culture supernatant, and other samples

Simplifed Process

Require only a standard magnetic rack, no special equipment needed

High Throughput

Enable simultaneous processing of multiple samples and support automated workflows

Isolated Exosome Validation

Exosomes isolated with Procell Micro Exosome Isolation Kit (Cat. No.: P-CA-501) have been validated by Transmission Electron Microscopy (Figure A), Western Blot (Figure B), and Nanoparticle Tracking Analysis (Figure C), demonstrating intact morphology, high purity, and a size distribution consistent with exosome characteristics.

Properties and Applications

Product Information	Product Name	Micro Exosome Isolation Kit	Universal Exosome Isolation Kit		Spin Column Exosome Isolation Kit	Exosome Isolation Kit (Precipitation)
Product Information	Cat. No.	P-CA-501	P-CA-502	P-CA-503	P-CA-504	P-CA-505
Kit Properties	Specificity/Purity
	Yield
	Fast and Convenient
	Cost-effectiveness
	Flux
	Automation
Sample Types	Micro Samples (Cerebrospinal Fluid, Tears, Tissue Fluid, etc.)
	Complex Samples (Serum, Plasma, Dairy Products, etc.)
	Large Volume Samples (Urine, Culture Supernatant, etc.)
	Plant Samples
	Tissue Samples
	Bacterial Samples
	Cell Culture Supernatant
Application Types	Western Blot
	Particle Size/Number Measurement (NTA, Nano-Flow Cytometry, etc.)
	TEM/Immunoelectron Microscopy
	qPCR/RNA-seq
	Protein Spectrum
	Cell/Animal/Drug-loading Experiments
	Exosome Targeted Modification

FAQs

Are the Exosome Isolation Kits suitable for both human and mouse samples?
Answer
Yes. The Procell Exosome Isolation Kits are not species-restricted and can be used with samples from humans, mice, and other mammalian species.
How can exosomes be isolated from 50 mL of bacterial culture supernatant obtained after high-speed centrifugation? Are there any recommended kits?
Answer

P-CA-502 or P-CA-503 can be used for exosome isolation.

Due to the large sample volume, it is recommended to concentrate the supernatant prior to isolation. A 50 kDa ultrafiltration device may be used for sample concentration; please refer to the manufacturer’s instructions for detailed procedures.

Alternatively, P-CA-505 can be used for simultaneous sample concentration and exosome isolation.
What target is used on the magnetic beads in the Micro Exosome Isolation Kit (Cat. No.:P-CA-501)?
Answer

The magnetic beads in this kit are functionalized to bind Phosphatidylserine (PS).

PS is an important phospholipid that is normally located on the inner leaflet of the cell membrane. Exosomes, as nano-sized vesicles secreted by cells, are enriched in PS on their membrane surface, making PS an effective target for exosome isolation and enrichment.

It should be noted that this method may also co-enrich membrane fragments derived from lysed cells.
Which exosome isolation kit is recommended for human umbilical cord mesenchymal stem cell-derived exosomes?
Answer

The choice of kit depends on the downstream application:

P-CA-501 is recommended when extremely high exosome purity is required, such as for high-sensitivity molecular detection or omics analyses.

P-CA-502/P-CA-503 are recommended for obtaining exosomes with high purity, intact structure, and well-preserved biological activity, suitable for cell function studies and drug delivery applications.

P-CA-504 is recommended when experimental time is limited and the requirements for exosome purity and yield are relatively moderate.

P-CA-505 is suitable for large sample volumes and cost-sensitive applications where downstream experiments do not require high purity. Please note that precipitation-based methods may co-precipitate impurities and potentially affect exosome properties.

It is recommended to select the appropriate kit based on the sample type and downstream experimental objectives.