Manual
P-CA-501
$ 648.00
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Product Introduction
Background
This kit utilizes our proprietary homogeneous liquid magnetic beads, engineered for the selective capture of exosomes while minimizing the adsorption of contaminants. This results in the isolation of exosomes with superior purity and high recovery rates. With its user-friendly design, exceptional purity, and robust recovery efficiency, it is ideally suited for exosome isolation from complex biological matrices such as plasma and serum. The isolated exosomes are versatile for a wide range of downstream applications, including Western blot (WB) analysis, nanoparticle tracking analysis (NTA), nanoparticle flow cytometry for particle size characterization, electron microscopy, omics profiling, and functional studies in cellular and animal models.
Separation Method
PS (Phosphatidylserine)-Affinity Magnetic Beads Method
Product Features
1 .Low Starting Volume: Enables the enrichment of exosomes from limited or precious samples.
2. Rapid Processing: Exosome isolation can be completed within 2 hours.
3. Ease of Use: Requires only a standard magnetic rack; no specialized equipment is needed.
4. High Reproducibility: Minimal operational proficiency required, ensuring consistent results.
5. High Recovery Rate: Achieves a recovery rate exceeding 90%.
6. High Purity: Purified exosomes are suitable for direct RNA and protein extraction.
7. Excellent Integrity: Magnetic bead-based isolation preserves exosome integrity better than other methods.
8. High Throughput: Capable of processing multiple samples simultaneously and compatible with automated systems.
9. Wide Applicability: Suitable for isolating exosomes from complex samples like plasma and serum, as well as simpler samples such as cell culture supernatants and urine.
Specifications
| Volume | 20Tests/50Tests |
| Acteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Green_Features | Sustainable packaging |
| Valid Period | 18 months |
| Storage Condition | This product can be stored at 2-8℃ for 18 months with shading light. |
| Shipping Condition | Ice bag |
Sample Processings
1.Sample Processing
1) Cell Removal: Centrifuge the sample at 300 × g for 5 minutes at 4℃. Carefully transfer the supernatant to a new centrifuge tube.
Note: This step can be skipped for cell-free samples.
2) Removal of Cellular Debris: Centrifuge the supernatant obtained from Step 1 at 2,000 × g for 10 minutes at 4°C. Transfer the supernatant to a new centrifuge tube.3) Removal of Large Particles: Centrifuge the supernatant obtained from Step 2 at 14,000 × g for 30 minutes at 4℃. Carefully transfer the resulting supernatant to a new centrifuge tube.
2. Exosomes Enrichment
1) Magnetic Bead Preparation: Add 100 μL of Exosome Beads to a 1.5 mL centrifuge tube. Place the tube on a magnetic rack for 1 minute and discard the storage buffer.
2) Sample Incubation: Add 500 μL of the preprocessed sample to the magnetic beads (if the volume is less than 500 μL, top up with Solution A). Secure the centrifuge tube on an orbital shaker and incubate at room temperature with gentle rotation (17 rpm) for 30 minutes.
Note: During the binding process, magnetic beads may aggregate in certain samples. This will not affect the separation efficiency.
3) Separation of Beads: Place the tube on a magnetic rack and let it stand for 30 seconds. Discard the supernatant.
4) Washing: Add 1 mL of Solution A and mix by gentle inversion. Place the tube on the magnetic rack, let it stand for 30 seconds, and discard the supernatant.
5) Repeat Washing: Repeat Step 4 two more times, retaining the magnetic beads at the end.
3. Exosome Isolation
Elution: Add 100 μL of Elution Buffer to the magnetic beads containing the enriched exosomes. Mix by pipetting.
1) Incubation: Secure the centrifuge tube on an orbital shaker and incubate at room temperature with gentle rotation (17 rpm) for 30 minutes.
2) Separation of Exosomes: Place the tube on a magnetic rack and let it stand for 30 seconds. Carefully transfer the supernatant to a new 1.5 mL centrifuge tube. This supernatant contains the isolated exosomes.
Note: Exosomes should not be stored in Elution Buffer for extended periods. For long-term storage, use ultrafiltration tubes (MWCO: 10 kDa) to replace the buffer with PBS.
Kit Components
| Name | Size | Appearance | Storage and Expiration Dat |
| Exosome Beads | 20Tests/50Tests | 2 mL/5 mL | 2-8℃, shading light |
| Solution A | 20Tests/50Tests | 100 mL/125 mL×2 | 2-8℃, shading light |
| Elution Buffer | 20Tests/50Tests | 2 mL/5 mL | 2-30℃, shading light |
FAQs
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Q:1 Which antibodies are conjugated to the magnetic beads used in this micro-volume exosome isolation kit (magnetic bead-based method)?
AnswerThe magnetic beads are functionalized to bind phosphatidylserine (PS)-associated proteins. Phosphatidylserine is a key phospholipid typically located on the inner leaflet of the cell membrane. Exosomes, as nanoscale vesicles secreted by cells, are also enriched in PS on their membrane surface, making it a useful target for exosome isolation and detection. However, it’s important to note that PS is also present on cell debris generated by lysis, which may lead to co-enrichment of non-exosomal material.
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Q:2 Which exosome isolation kit is recommended for extracting exosomes from human umbilical cord mesenchymal stem cells?
AnswerIf extremely high exosome purity is require, for example, for downstream high-sensitivity molecular analyses, kit P-CA-501 is recommended. For applications requiring high-purity, structurally intact, and biologically active exosomes, such as cell function studies or drug delivery, kits P-CA-502 or P-CA-503 are suitable. If experimental is time - sensitive and high purity or yield is not essential, kit P-CA-504 is an efficient option. For large sample volumes where cost control is a concern and purity demands are moderate, kit P-CA-505 (precipitation-based) can be used. However, note that precipitation may co-isolate impurities and the reagents could potentially affect exosome integrity. It is recommended to choose a kit based on the specific requirements of your experiment.
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Q:3 Is the exosome isolation kit compatible with both human and mouse samples?
AnswerYes, it is universal and has no species restrictions.
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Q:4 What is the difference between the two universal SEC exosome isolation kits P-CA-502 and P-CA-503?
AnswerThe specifications are different, and the columns themselves are also slightly different (the 502 column is smaller), but the usage is the same. The sample volume of P-CA-502 is 1mL, and the sample volume of P-CA-503 is 3mL.
