Mergene1000^® Hep G2 Cell-Specific siRNA Transfection Reagent

Product Introduction

Background

Mergene1000^® Hep G2 Cell-Specific siRNA Transfection Reagent is a new and stable siRNA transfection reagent with efficient RNA compression ability. It can quickly and efficiently transfect RNA into cells, while forming a stable complex to effectively protect RNA from nuclease degradation. It is specifically formulated for use with Hep G2 cells, achieving high transfection efficiency. The reagent is distinguished by its low toxicity, excellent stability, ease of operation, and high reproducibility.

Matters Need Attention

1. The cell inoculation amount and transfection ratio provided above are based on experiments conducted with Hep G2 cells and are for reference only. The specific experimental dosage should be adjusted according to the actual conditions.
2. The product is transported with ice bag and stored at 2-8°C. It can be aliquoted and stored upon use to avoid multiple prolonged openings of the lid.
3. MEM, with NEAA medium should be prepared separately for the dilution of siRNA and transfection reagents.
4. During transfection, ensure that the degree of cell confluence is not less than 50%, and it is generally maintained at around 50% to 70%. The specific plating density can be adjusted according to the actual conditions of the cells.
5. After transfection, there is no need to remove the transfection complex or replace with fresh culture medium. The actual operation can be based on the cell status, after transfection culture 4-6 hours to choose to change the medium.
6. The use of high purity siRNA is helpful to obtain higher transfection efficiency. Before transfection, ensure that siRNA gene silencing expression does not affect cell viability.
7. The experimental process utilized RNA-free and pyrogen-free materials, such as centrifuge tubes, pipette tips, and buffers.
8. For in vitro transfection and research use only.
9. For your safety and health, please wear experimental clothes and wear disposable gloves aseptic operation.

System Certification

GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Mergene1000^® Hep G2 Cell-Specific siRNA Transfection Reagent complies with ISO 9001.