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CFSE Cell Division Tracker Kit

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Cat. No.: P-CA-345

P-CA-345

$ 458.00

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Product Introduction

Detection Principle CFDA SE is a kind of fluorescein dye with membrane permeability, which has no fluorescence itself. When CFDA SE enters living cells through the cell membrane, it can be catalyzed by esterase in cytoplasm to produce carboxyfluorescein succinimide ester (CFSE), which can emit strong green fluorescence, can not penetrate the cell membrane, and can remain intact in the cell. CFSE can also spontaneously and irreversibly combine with amino groups of intracellular proteins to couple to these proteins. At the same time, excessive and uncoupled CFDA SE is passively diffused back into extracellular medium and removed by subsequent washing steps.
Detection Method Fluorometric method
Sample Type Cell samples
Detection Instrument Flow Cytometer;Fluorescence Microscope
Dye Type CFSE
Ex/Em 494/521
Channel Set FITC
Other Reagents Required PBS(PB180327)
Storage This product can be stored at -5~-20°C for 12 months with shading light.
Expiration Date 12 months
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