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CL-0311
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | 293E |
| Synonyms | 293 c18;293c18;HEK 293 c18;HEK-293 c18;HEK293-EBNA1;HEK-293-EBNA;HEK 293-EBNA;HEK 293 EBNA;HEK293EBNA;293 EBNA;293-EBNA1;293-EBNA;293/EBNA;293EBNA;EBNA-293;EBNA293;293E;HEK293E;HEK/EBNA;HEK-EBNA;HEK.EBNA;293/EBNA-1 |
| Cellosaurus Accession | CVCL_6974 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like; polygonal |
| Tissue | Embryonic kidney |
| Age | Fetus |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | IMDM [PM150510]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:5 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | 293E cells are derived from HEK-293 cells and engineered to stably express Epstein-Barr virus nuclear antigen 1 (EBNA1). |
| Biosafety Level | BSL-2 |
Documents
MSDSPublications
Asymmetric pendrin homodimer reveals its molecular mechanism as anion exchanger
Journal: Nature Communications (2023) IF: 16.6
DOI: 10.1038/s41467-023-38303-0
Product Cited: 293E Cells Line
