293T/17

Name: 293T/17
Brand: Procell
SKU: CL-0469
Price: 460.0 USD
Availability: InStock

Copy the product info.

Cat. No.: CL-0469

Publications(18)

CL-0469

$ 460.00

Select a size

1×10^6Cells/Vial×2Vials

Quantity

- +

In stock

Inquire

For order requirements and technical needs, please Contact Us

Product Introduction Documents Publications

Product Introduction

General information

Cell Name	293T/17
Synonyms	HEK 293T/17;293T/17
Cellosaurus Accession	CVCL_1926
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Kidney
Age	Fetus
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line exhibits weak adherence, so all manipulations must be carried out gently. Pre-warm the culture medium before medium exchange. Serum-free non-programmed cryopreservation medium is recommended for cell cryopreservation.
Complete Medium	DMEM [PM150210]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:6
Medium Renewal	2 to 3 times per week
Dissociation Duration	0.5-1 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

The 293T/17 cell line is a derivative of the 293T cell line. 293T is a highly transfectable derivative of the 293 [HEK-293] cell line, generated by inserting the temperature-sensitive gene for the SV40 T-antigen. 293T cells were cloned and tested with the pBND and pZAP vectors, yielding a derivative, designated 293T/17, capable of producing high titers of infectious retrovirus. 293T/17 cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability. 293T/17 cells were transfected with the pCRIPenv- and pCRIPgag-2 vectors to generate the ANJOU65 cell line. ANJOU65 cells were further transfected with the pCRIPgag-2 and pGPT2E vectors to obtain the ecotropic envelope-expressing packaging cell line BOSC23. ANJOU65 cells were also transfected with the pCRIPAMgag vector carrying the gpt resistance gene to generate the amphotropic envelope-expressing packaging cell line Bing.

Biosafety Level

BSL-2