293T [HEK-293T]

Name: 293T [HEK-293T]
Brand: Procell
SKU: CL-0005
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0005

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CL-0005

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	293T [HEK-293T]
Synonyms	Hek293T;HEK-293T;HEK 293T;HEK-293-T;HEK 293 T;293-T;293 T;293T;Human Embryonic Kidney 293T;293tsA1609neo
Cellosaurus Accession	CVCL_0063
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Embryo; kidney
Age	Fetus
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line exhibits weak adherence, so all manipulations must be carried out gently. Pre-warm the culture medium before medium exchange. Serum-free non-programmed cryopreservation medium is recommended for cell cryopreservation.
Complete Medium	DMEM [PM150210]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:6
Medium Renewal	2 to 3 times per week
Dissociation Duration	0.5-1 min
Thawing Procedure	若收到细胞大片脱落，请按照如下处理方式处理：<br/>1. 将培养瓶内所有培养基转入无菌离心管，离心收集细胞（1200 rpm 3 min）去除旧培养基；<br/>2. 用PBS重悬细胞，将所有细胞收集到一个离心管中，再次离心（1200 rpm 3 min）去除PBS；<br/>3. 加入1 mL左右0.25%胰酶重悬细胞，混匀即可，不能吹打太多次，放入培养箱消化3分钟。<br/>4. 消化好后，用移液枪轻轻吹打细胞悬液，使细胞团分散，迅速加入3-5 mL含血清的培养基混匀以终止消化，离心（1200 rpm 3 min）去除胰酶；<br/>5. 加入5 mL左右的细胞相应的完全培养基混匀，按比例接入无菌培养瓶/皿中（首次传代推荐1:3）。
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The 293T [HEK-293T] cell line is a highly transfectable derivative of the 293 [HEK-293] cell line, generated by inserting the temperature-sensitive gene for the SV40 T-antigen.
Biosafety Level	BSL-1