2V6.11
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Cat. No.: CL-0314
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CL-0314
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | 2V6.11 |
| Cellosaurus Accession | CVCL_6355 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Tissue | Kidney |
| Age | Fetus |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:5 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 1-2 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The 2V6.11 cell line was derived from the human embryonic kidney cell line 293 (HEK-293) in 2001. 293 cells were transfected with plasmid pVgRXR, which carries a zeocin-resistance marker, to generate 293pVgRXR cells. Subsequently, these cells were transfected with plasmid pEKORF6 containing the Ad5 E4ORF6 gene encoding the E4 34 kDa protein. pEKORF6 was derived from plasmid pIndHydro, which contains a hygromycin resistance gene and SV40 viral sequences. The 2V6.11 cell line was isolated by picking single clones using cloning rings in medium containing hygromycin. Induced expression of the human adenovirus E4 34 kDa protein in 2V6.11 cells can be detected by immunoblotting. This cell line can be used as a tool for studying the adenovirus E4 oncogene. |
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