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Akata

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Cat. No.: CL-0763 Publications(1)
Akata - 1
  • AkataAkata - 1
  • AkataAkata - 2
  • AkataAkata - 3
  • +2

CL-0763

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction

General information
Cell Name Akata
Synonyms AKATA;Akata-BL;Akata BL;Akata-EC;Akata-Early Culture
Cellosaurus Accession CVCL_0148
Organism Human
Growth Properties Suspension
Morphology Lymphoblast-like
Disease lymphoma cells
Tissue Peripheral blood
Age 4Y
Sex Male
Instructions 1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere Air, 95%; CO₂, 5%
Temperature 37℃
Subculturing Procedure Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium 90% FBS+10% DMSO
Storage Conditions For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150 °C to −196 °C. Storage at −80 °C is restricted to short-term interim use only.
Background Akata is an Epstein‑Barr virus (EBV)‑producing cell line derived from a Japanese patient with Burkitt’s lymphoma. It is also designated as Akata EC.
Biosafety Level BSL-2

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