ATDC-5

FAQs

• Q:1 Why does Procell specify its cell line products as 1×10^6 cells × 2 vials? Compared with some other manufacturers that provide larger cell counts, what are the advantages of your products?

  Answer

  The total cell count of 2×10^6 cells (1×10^6 Cells/Vial × 2 Vials) is set in accordance with industry standards established by leading international cell banks such as ATCC. We adopt this specification to ensure that the condition of delivered cells aligns with international standards. More importantly, we firmly believe that cell quality is far more important than sheer cell count. All the 2×10^6 cells (1×10^6 Cells/Vial × 2 Vials) we specify are highly viable and highly pure. Furthermore, most of the cell lines we provide support multiple passages. This means that as long as the cells remain healthy, the 2×10^6 high-quality cells you receive will be more than sufficient to support all your subsequent experiments. In the long run, minor variations in the initial cell count will have virtually no impact on the entire experimental cycle. In addition, our unique packaging design offers you extra assurance: we aliquot the 2×10^6 cells into two cryovials. Even if the first thawing procedure is unsuccessful, you still have a second chance to try, which eliminates the risk of losing all cells due to a single operational error and provides double protection for your experiments. When you choose Procell, you gain more than just 2×10^6 cells—you get a complete, worry-free research solution: we provide free STR typing for every cell line to ensure zero-risk experimentation; we offer rapid delivery to save you valuable time; our dedicated technical support team is always available to answer your questions and guide you throughout the entire process; and we provide specialized complete culture media for ready-to-use convenience. Therefore, our 2×10^6 cells (1×10^6 Cells/Vial × 2 Vials) are an identity-verified, viability-guaranteed, ready-to-use, and worry-free "accelerator" for your research. Our focus is helping you obtain experimental results faster and more reliably—not just increasing the number of cells we provide. We look forward to becoming your most trusted cell supplier.

• Q:2 Why do the cell culture conditions specified in the literature I reviewed differ from those provided on your official website?

  Answer

  Certain cell lines do show variability in their acceptable culture conditions. Our company prioritizes the culture conditions specified by the original source provider and the investigator who established the cell line. Variations in culture conditions may arise from modifications made by different laboratories during long-term preservation. To prevent adaptation problems after changing culture conditions, we recommend following the manufacturer-recommended culture conditions for cultivation.

• Q:3 The color of cryopreserved cells varies, with some appearing pink and others yellowish. Does this variation affect their viability after thawing?

  Answer

  This phenomenon frequently occurs between different cryopreservation batches, and is associated with cell density, cryopreservation solution composition, and pH changes induced by temperature variations. This discoloration occurs only occasionally, and does not necessarily indicate compromised cell viability. We recommend attempting cell resuscitation first to assess their condition.

• Q:4 Are the RRIDs of cells from the same strain obtained from different sources all the same?

  Answer

  Yes, each cell line has only one unique RRID.

• Q:5 How can I improve the survival rate of cryopreserved cells stored at -80°C?

  Answer

  When storing cryovials, place them in the innermost compartment of the refrigerator's freezer section; avoid placing them on the door shelves or near the refrigerator opening. Additionally, we recommend using refrigerators that are opened less frequently to minimize temperature fluctuations caused by opening and closing the door.

• Q:6 How long can cells be preserved in a -80°C freezer?

  Answer

  The preservation duration of cells at -80°C depends on the operating frequency of the refrigerator and the temperature sensitivity of the cells themselves; therefore, there is no standardized storage period. It is recommended to transfer the cells into liquid nitrogen for long-term storage as early as possible.

• Q:7 For frozen cells transported via dry ice, should they be stored at -80°C or in liquid nitrogen upon receipt?

  Answer

  It is recommended to immediately transfer the received frozen cells into liquid nitrogen for long-term storage; storage at -80℃ should only be used as a short-term interim solution. This is because at -80℃, cellular metabolism is significantly slowed but not completely halted. In addition, frequent opening and closing of the -80℃ freezer causes temperature fluctuations that reduce cell viability.

• Q:8 My cells are growing very slowly. What could be the cause of this?

  Answer

  Please see some common reasons below, and solutions to the issue: - Growth medium is not correct: Use pre-warmed growth medium as recommended by the supplier. - Serum in the growth medium is of poor quality: Use serum from a different lot. and choose good quality serum to ensure nutrition. - Cells have been passaged too many times: Use healthy, low-passage number cells. - Cells were allowed to grow beyond confluency: Passage mammalian cells when they are in the log-phase before they reach confluency. - Culture is contaminated with mycoplasma: Discard cells, media, and reagents. Obtain new stocks of cells, and use them with fresh media and reagents.

• Q:9 What factors can contribute to rapid cell death/culture failure?

  Answer

  There are a number of events that can contribute to this: 1. Incorrect CO2 levels:Monitor the level of CO2 manually with a Fyrite kit, available from Bacharach. Check if the manual readings concur with the readings displayed on the incubator. If the incubator has a trace readout, check the printout for fluctuations in CO2 level. Check the settings to ensure that CO2 levels are set at appropriate levels for your cell line (usually between 5 and 10%). Check line connections frequently for leaks. Avoid frequent opening and closing of incubator doors. 2. Temperature fluctuations in the incubator:Monitor the temperature of incubator with a good thermometer inside the incubator. 3. Amphotericin B or other preventive antibiotics/antimycotics are present at toxic concentrations:Use at recommended levels. 4. Humidity is incorrect:Check the water level in the water pan. Humidity is vital to appropriate gas exchange for many types of cells and media. 5. Incorrect osmotic pressure in medium:Check osmolality of complete medium. Most mammalian cells can tolerate an osmolality of 260 to 350 mOsm/kg. Additions of reagents such as HEPES and drugs may affect osmolality. 6. Contamination by microorganisms:Bacterial and fungal contaminations are usually easily visible; symptoms of mycoplasma contamination are more subtle, and careful monitoring of culture morphology and regular testing are necessary to detect this type of contamination. 7. Inappropriate medium is being used:Double-check that the medium used is appropriate for your cell type and culture application. For example, ensure that the medium being used for serum-free culture is actually designed for serum-free culture; make sure that appropriate selective drugs are used at appropriate levels; check the expiration dates for the reagents being used; and store medium at appropriate temperatures in the dark.