BC-3
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Cat. No.: CL-0713
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CL-0713
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | BC-3 |
| Synonyms | BC3 |
| Cellosaurus Accession | CVCL_1080 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | lymphoma cells |
| Tissue | Pleural effusion |
| Age | 85Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+20% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | Every 3 to 4 days |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | BC-3 is a human B-cell non‑Hodgkin lymphoma cell line established in 1995 from the pleural effusion of an 85‑year‑old HIV‑negative male patient diagnosed with primary effusion lymphoma (PEL). The cell line is positive for human herpesvirus 8 (HHV‑8 / KSHV) and negative for Epstein‑Barr virus (EBV). |
| Biosafety Level | BSL-2 |
MSDS
