bEnd.3 [BEND3]

Name: bEnd.3 [BEND3]
Brand: Procell
SKU: CL-0598
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0598

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CL-0598

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1×10^6Cells/Vial×2Vials

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Product Introduction

General Information

Cell Name	bEnd.3 [BEND3]
Synonyms	bEND.3;b.End3;Bend.3;bEnd3;brain-derived Endothelial cells.3
Cellosaurus Accession	CVCL_0170
Organism	Mouse
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Brain
Age	6W
Sex	Sex unspecified

Handling Information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. At low density, cells have irregular morphology, low cell number, and a large spreading area. At high density, morphology becomes more uniform and cell bodies are dense. 4. Cell proliferation is relatively slow. A 1:2 split ratio is recommended for the first subculture. After cells stabilize, the subculture split ratio should also not exceed 1:4. 5. Monitor the color of the culture medium; a purplish color will cause increased cell floating. 6. Dissociation time varies with cell growth duration and cell density. It should be determined based on cell condition during dissociation. Terminate dissociation only when cells can be dislodged.
Complete Medium	DMEM [PM150210]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-8 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference Materials

Background	bEnd.3 [BEND3] is a mouse brain microvascular endothelial cell line isolated from endothelioma-bearing mouse brain tissue and immortalized via retroviral transduction of polyomavirus middle T antigen. bEnd.3 cells display canonical endothelial phenotypes, such as von Willebrand factor expression and the capacity to internalize low-density lipoprotein (LDL). bEnd.3 cells constitutively produce intercellular adhesion molecule 1 (ICAM-1), and ICAM-1 expression is further elevated following exposure to LPS, IL-1, and TNF-α. Early-passage bEnd.3 cells express surface MAdCAM-1 under basal conditions, yet this marker disappears beyond passage 30. bEnd.3 cells also constitutively express vascular cell adhesion molecule 1 (VCAM-1) prior to passage 30, with VCAM-1 expression undetectable at later passages. Tumor necrosis factor-α (TNF-α) triggers P-selectin expression in bEnd.3 cells, and this inductive effect is more pronounced in cells cultured past passage 30. bEnd.3 cells serve as a standard in vitro model for blood-brain barrier and neurovascular biology investigations.
Antigen Expression	ICAM-1 +; VCAM-1 +; MAdCAM-1 +
Gene Expression	von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL). The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd3.
Biosafety Level	BSL-1

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Publications

Efficient amyloid-β degradation in Alzheimer’s disease using SPYTACs

Journal: CELL (2026) IF: 42.5

DOI: 10.1016/j.cell.2026.01.034

Product Cited: HuH-7 Cells Line, U251 Cells Line, BV2 Cells Line, bEnd.3 Cells Line, Neuro-2a Cells Line, SH-SY5Y Cells Line, AML12 Cells Line, C8-D1A Cells Line

Adrenomedullin Antagonist Nanoparticles Inhibit Breast Cancer Brain Metastasis by Immunomodulating Monocytes/Macrophages

Journal: ACS Nano (2026) IF: 16

DOI: 10.1021/acsnano.5c15040

Product Cited: bEnd.3 Cells Line

Dual-stiffness nanoparticles for compartment-specific drug delivery in stroke

Journal: Nature Communications (2026) IF: 15.7

DOI: 10.1038/s41467-026-70340-3

Product Cited: bEnd.3 Cells Line, RPMI-1640 Medium, DMEM (High glucose) Medium

Endothelial Cell-Based Vascular Bandages for Blood–Brain Barrier Repair and Targeted siRNA Delivery

Journal: Advanced Science (2026) IF: 14.1

DOI: 10.1002/advs.202517780

Product Cited: bEnd.3 Cells Line

An Activity-Dependent NEPAS–PTX3 Axis Links Neurovascular and Myelin Deficits to Cognitive Impairment

Journal: Advanced Science (2026) IF: 14.1

DOI: 10.1002/advs.202521069

Product Cited: bEnd.3 Cells Line

FAQs

Q:1 Why does Procell specify its cell line products as 1×10^6 cells × 2 vials? Compared with some other manufacturers that provide larger cell counts, what are the advantages of your products?
Answer
The total cell count of 2×10^6 cells (1×10^6 Cells/Vial × 2 Vials) is set in accordance with industry standards established by leading international cell banks such as ATCC. We adopt this specification to ensure that the condition of delivered cells aligns with international standards. More importantly, we firmly believe that cell quality is far more important than sheer cell count. All the 2×10^6 cells (1×10^6 Cells/Vial × 2 Vials) we specify are highly viable and highly pure. Furthermore, most of the cell lines we provide support multiple passages. This means that as long as the cells remain healthy, the 2×10^6 high-quality cells you receive will be more than sufficient to support all your subsequent experiments. In the long run, minor variations in the initial cell count will have virtually no impact on the entire experimental cycle. In addition, our unique packaging design offers you extra assurance: we aliquot the 2×10^6 cells into two cryovials. Even if the first thawing procedure is unsuccessful, you still have a second chance to try, which eliminates the risk of losing all cells due to a single operational error and provides double protection for your experiments. When you choose Procell, you gain more than just 2×10^6 cells—you get a complete, worry-free research solution: we provide free STR typing for every cell line to ensure zero-risk experimentation; we offer rapid delivery to save you valuable time; our dedicated technical support team is always available to answer your questions and guide you throughout the entire process; and we provide specialized complete culture media for ready-to-use convenience. Therefore, our 2×10^6 cells (1×10^6 Cells/Vial × 2 Vials) are an identity-verified, viability-guaranteed, ready-to-use, and worry-free "accelerator" for your research. Our focus is helping you obtain experimental results faster and more reliably—not just increasing the number of cells we provide. We look forward to becoming your most trusted cell supplier.
Q:2 Why do the cell culture conditions specified in the literature I reviewed differ from those provided on your official website?
Answer
Certain cell lines do show variability in their acceptable culture conditions. Our company prioritizes the culture conditions specified by the original source provider and the investigator who established the cell line. Variations in culture conditions may arise from modifications made by different laboratories during long-term preservation. To prevent adaptation problems after changing culture conditions, we recommend following the manufacturer-recommended culture conditions for cultivation.
Q:3 The color of cryopreserved cells varies, with some appearing pink and others yellowish. Does this variation affect their viability after thawing?
Answer
This phenomenon frequently occurs between different cryopreservation batches, and is associated with cell density, cryopreservation solution composition, and pH changes induced by temperature variations. This discoloration occurs only occasionally, and does not necessarily indicate compromised cell viability. We recommend attempting cell resuscitation first to assess their condition.
Q:4 Are the RRIDs of cells from the same strain obtained from different sources all the same?
Answer
Yes, each cell line has only one unique RRID.
Q:5 How can I improve the survival rate of cryopreserved cells stored at -80°C?
Answer
When storing cryovials, place them in the innermost compartment of the refrigerator's freezer section; avoid placing them on the door shelves or near the refrigerator opening. Additionally, we recommend using refrigerators that are opened less frequently to minimize temperature fluctuations caused by opening and closing the door.
Q:6 How long can cells be preserved in a -80°C freezer?
Answer
The preservation duration of cells at -80°C depends on the operating frequency of the refrigerator and the temperature sensitivity of the cells themselves; therefore, there is no standardized storage period. It is recommended to transfer the cells into liquid nitrogen for long-term storage as early as possible.
Q:7 For frozen cells transported via dry ice, should they be stored at -80°C or in liquid nitrogen upon receipt?
Answer
It is recommended to immediately transfer the received frozen cells into liquid nitrogen for long-term storage; storage at -80℃ should only be used as a short-term interim solution. This is because at -80℃, cellular metabolism is significantly slowed but not completely halted. In addition, frequent opening and closing of the -80℃ freezer causes temperature fluctuations that reduce cell viability.
Q:8 My cells are growing very slowly. What could be the cause of this?
Answer
Please see some common reasons below, and solutions to the issue: - Growth medium is not correct: Use pre-warmed growth medium as recommended by the supplier. - Serum in the growth medium is of poor quality: Use serum from a different lot. and choose good quality serum to ensure nutrition. - Cells have been passaged too many times: Use healthy, low-passage number cells. - Cells were allowed to grow beyond confluency: Passage mammalian cells when they are in the log-phase before they reach confluency. - Culture is contaminated with mycoplasma: Discard cells, media, and reagents. Obtain new stocks of cells, and use them with fresh media and reagents.
Q:9 What factors can contribute to rapid cell death/culture failure?
Answer
There are a number of events that can contribute to this: 1. Incorrect CO2 levels:Monitor the level of CO2 manually with a Fyrite kit, available from Bacharach. Check if the manual readings concur with the readings displayed on the incubator. If the incubator has a trace readout, check the printout for fluctuations in CO2 level. Check the settings to ensure that CO2 levels are set at appropriate levels for your cell line (usually between 5 and 10%). Check line connections frequently for leaks. Avoid frequent opening and closing of incubator doors. 2. Temperature fluctuations in the incubator:Monitor the temperature of incubator with a good thermometer inside the incubator. 3. Amphotericin B or other preventive antibiotics/antimycotics are present at toxic concentrations:Use at recommended levels. 4. Humidity is incorrect:Check the water level in the water pan. Humidity is vital to appropriate gas exchange for many types of cells and media. 5. Incorrect osmotic pressure in medium:Check osmolality of complete medium. Most mammalian cells can tolerate an osmolality of 260 to 350 mOsm/kg. Additions of reagents such as HEPES and drugs may affect osmolality. 6. Contamination by microorganisms:Bacterial and fungal contaminations are usually easily visible; symptoms of mycoplasma contamination are more subtle, and careful monitoring of culture morphology and regular testing are necessary to detect this type of contamination. 7. Inappropriate medium is being used:Double-check that the medium used is appropriate for your cell type and culture application. For example, ensure that the medium being used for serum-free culture is actually designed for serum-free culture; make sure that appropriate selective drugs are used at appropriate levels; check the expiration dates for the reagents being used; and store medium at appropriate temperatures in the dark.