Ca Ski

Name: Ca Ski
Brand: Procell
SKU: CL-0048
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0048

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CL-0048

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	Ca Ski
Synonyms	Ca-Ski;CaSki;Caski;CASKI
Cellosaurus Accession	CVCL_1100
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	cervical cancer cells
Tissue	Uterus; Cervix; derived from metastatic site: small bowel mesentery
Age	40Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	6-8 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	Ca Ski cells were established from a metastasis in the small bowel mesentery. It has been reported that Ca Ski cells contain an integrated human papillomavirus type 16 genome (approximately 600 copies per cell) as well as HPV-18–related sequences.
Gene Expression	beta subunit of human chorionic gonadotropin (hCG); tumor associated antigen
Biosafety Level	BSL-2