CCK-81
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Cat. No.: CL-1112
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CL-1112
$ 460.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | CCK-81 |
| Synonyms | CCK81 |
| Cellosaurus Accession | CVCL_2873 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Tissue | Colon |
| Age | 62Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Dark granules and debris are present in this cell line; this is a normal phenomenon and can be removed by medium exchange. 4. This cell line grows in sheets; at high cell density, a small number of cells grow in stacked layers. 5. When dark granules and debris are abundant, the culture medium becomes turbid. |
| Complete Medium | MEM (ATCC modified) [PM150467]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | every 2 to 3 days |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | CCK-81 cells do not secrete immunoglobulin. They are resistant to 20 μg/mL 8-azaguanine but sensitive to HAT. This cell line can be used as a B-cell fusion partner for hybridoma production. It has been tested and confirmed negative for ectromelia virus (mousepox). |
| Biosafety Level | BSL-1 |
MSDS
