CCRF-CEM [CCRF CEM]

Name: CCRF-CEM [CCRF CEM]
Brand: Procell
SKU: CL-0058
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0058

Publications(15)

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	CCRF-CEM [CCRF CEM]
Synonyms	CCRF/CEM;CCRFCEM;CCRF.CEM;CCRF CEM;CCRF;CEM;CEM-CCRF;CEM-CCRF (CAMR);CCRF/CEM/0;CEM/0;CEM-0;CCRF-CEM/S;GM03671
Cellosaurus Accession	CVCL_0207
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	leukemia cells
Tissue	Peripheral blood
Age	4Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The CCRF-CEM [CCRF CEM] cell line is a lymphoblastoid cell line established by G.E. Foley et al. in November 1964 from the peripheral blood buffy coat of a 4-year-old Caucasian female with acute lymphoblastic leukemia.
Tumorigenic	Yes, in syrian hamsters.
Antigen Expression	CD3; Homo sapiens, expressed CD4; Homo sapiens, expressed CD5; Homo sapiens, expressed CD7; Homo sapiens, expressed
Gene Expression	CD3; Homo sapiens, expressed CD4; Homo sapiens, expressed CD5; Homo sapiens, expressed CD7; Homo sapiens, expressed
Biosafety Level	BSL-1