CoC1-DDP

Name: CoC1-DDP
Brand: Procell
SKU: CL-0065
Price: 720.0 USD
Availability: InStock

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Cat. No.: CL-0065

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1×10^6Cells/Vial×2Vials

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Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+0.5-2 μg/mL Supplement1+1% Supplement2
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	COC1-DDP cells are a cisplatin-resistant subline derived from COC1 cells, established by Professor Chen Huizhen and colleagues using a dose-escalating drug induction method. COC1-DDP cells exhibit 6.5-fold greater resistance to cisplatin (DDP, 1 μg/mL) than the parental COC1 cells, and also show varying degrees of cross-resistance to carboplatin (CBDCA) and mitomycin C (MMC). COC1-DDP cells can be used for in vitro experimental studies of ovarian cancer therapy.
Tumorigenic	Yes, forms tumors in nude mice.
Biosafety Level	BSL-1