COLO 205

Name: COLO 205
Brand: Procell
SKU: CL-0066
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0066

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	COLO 205
Synonyms	Colo 205;CoLo 205;COLO-205;Colo-205;COLO.205;Colo205;COLO205;Co 205
Cellosaurus Accession	CVCL_0218
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Epithelial-like
Disease	colorectal cancer cells
Tissue	Large intestine; Colon; derived from metastatic site: ascites
Age	70Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	every 2 to 3 days
Dissociation Duration	2-3 min
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Weakly adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. For firmly adherent cells, rinse with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 4. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The COLO 205 cell line was established in 1957 by T.U. Sample and colleagues from ascites of a 70-year-old Caucasian male with colon cancer. The patient had been treated with 5-fluorouracil for 4–6 weeks prior to ascites collection. COLO 205 cells are keratin-positive by immunoperoxidase staining.
Tumorigenic	Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells).
Gene Expression	carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10⁶ cells cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10), The cells are positive for keratin by immunoperoxidase staining.
Biosafety Level	BSL-1