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CL-0067
$ 460.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | COLO 320DM |
| Synonyms | COLO-320DM;COLO_320DM;COLO-320-DM;COLO320/DM;COLO320-DM;COLO320DM;Colo320DM;COLO320 DM;COLO 320 DM |
| Cellosaurus Accession | CVCL_0219 |
| Organism | Human |
| Growth Properties | Semi-adherent and semi-suspension |
| Morphology | Round |
| Disease | colorectal cancer cells |
| Tissue | Large intestine; Colon |
| Age | 55Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:6 |
| Medium Renewal | every 2 to 3 days |
| Dissociation Duration | 1-2 min |
| Subculturing Procedure | 1. This cell line displays mixed adherent and suspended phenotypes. Although suspended cells remain viable, their density is low and they may be aspirated and discarded directly. 2. Rinse the adherent cells with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 3. Seed the cells into fresh culture vessels at the recommended split ratio. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | COLO 320DM cells produce serotonin, norepinephrine, epinephrine, ACTH, and parathyroid hormone. COLO 320DM cells stain weakly positive for keratin and vimentin. |
| Tumorigenic | Yes, in nude mice. |
| Gene Expression | serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
m6A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression
Journal: Aging-US (2023) IF: 5.2
Product Cited: Human Colonic Mucosal Epithelial cells, RKO Cells Line, HCT 116 Cells Line, SW480 [SW-480] Cells Line, COLO 320DM Cells Line
