D283 Med

Name: D283 Med
Brand: Procell
SKU: CL-0271
Price: 420.0 USD
Availability: InStock

Copy the product info.

Cat. No.: CL-0271

Publications(2)

CL-0271

$ 420.00

Select a size

1×10^6Cells/Vial×2Vials

Quantity

- +

In stock

Inquire

For order requirements and technical needs, please Contact Us

Product Introduction Documents Publications

Product Introduction

General information

Cell Name	D283 Med
Synonyms	D283 MED;D283-MED;D-283 Med;D-283MED;D283MED;D283Med;D283-Med;D283;Med 283;H283
Cellosaurus Accession	CVCL_1155
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Epithelial-like
Tissue	Cerebellum; derived from metastatic site: ascites
Age	6Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line proliferates slowly and has a long recovery period after cryopreservation. Medium changes should be performed every 4 days. If predominantly adherent growth is desired during culture, follow this recovery protocol: When adherent density is below 60%, collect floating cells during medium changes and return them to the original flask. When adherent density exceeds 60%, floating cells may be discarded during medium changes. Subculture when adherent density reaches 80%.
Complete Medium	MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-4 min
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Although suspended cells remain viable, their density is low and they may be aspirated and discarded directly. 2. Rinse the adherent cells with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 3. Seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The D283 Med cell line was established in 1985 by Friedman et al. from the ascitic fluid of a 6-year-old Caucasian male with medulloblastoma and peritoneal metastasis.
Tumorigenic	Yes, in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.) Yes, in soft agar.
Gene Expression	The cells produce tumors in nude mice and the resulting tumors are glutamine synthetase positive; neuron specific enolase positive; glial fibrillary acidic proteins negative; S100 (S-100) protein negative; The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase; the brain isoenzyme of creatine kinase; L-DOPA decarboxylase; bombesin-like immunoreactivity)
Biosafety Level	BSL-1