D341Med

Name: D341Med
Brand: Procell
SKU: CL-0683
Price: 420.0 USD
Availability: InStock

Copy the product info.

Cat. No.: CL-0683

Publications(1)

CL-0683

$ 420.00

Select a size

1×10^6Cells/Vial×2Vials

Quantity

- +

In stock

Inquire

For order requirements and technical needs, please Contact Us

Product Introduction Documents Publications

Product Introduction

General information

Cell Name	D341Med
Synonyms	D-341 Med;D-341 MED;D-341Med;D-341MED;D341_Med;D341Med;D341MD;D-341;D341;Med 341;H341
Cellosaurus Accession	CVCL_0018
Organism	Human
Growth Properties	Aggregated suspension with loose adherence
Morphology	Lymphoblast-like
Tissue	Brain
Age	3.5Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	MEM (ATCC modified) [PM150467]+10% Nutrient+1 mM Supplement1+1% Supplement2
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-4 min
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Weakly adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. For firmly adherent cells, rinse with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 4. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma.
Biosafety Level	BSL-1