Dakiki
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Cat. No.: CL-1172
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CL-1172
$ 600.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | Dakiki |
| Synonyms | DAKIKI;DAKIKI Clone 1 |
| Cellosaurus Accession | CVCL_3675 |
| Organism | Human |
| Growth Properties | Suspension with multicellular aggregation |
| Morphology | Lymphoblast-like |
| Tissue | Lymph node |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line grows in aggregates, and the presence of scattered single cells surrounding the clusters is a normal phenomenon. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Antigen Expression | IgA, lambda light chainIg |
| Gene Expression | immunoglobulin, monoclonal antibody |
| Biosafety Level | BSL-2 |
MSDS
