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Product Introduction
| Cell Name | Daudi |
| Synonyms | DAUDI;NK-10A;NK-10a;NK 10a;NK10a;GM03190;GM3190;GM17346 |
| Cellosaurus Accession | CVCL_0008 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | lymphoma cells |
| Tissue | Peripheral blood |
| Age | 16Y |
| Sex | Male |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | The Daudi cell line was established in May 1967 by E. Klein and G. Klein from a 16-year-old Black male patient with Burkitt's lymphoma. Daudi cells are positive for surface immunoglobulin (sIg⁺) and EBNA/viral capsid antigen (VCA), but negative for β2-microglobulin. The cell line harbors Epstein-Barr virus (EBV). Daudi is a typical B-lymphoblast cell line and is widely used in studies of lymphomagenesis, as well as EBV biology. |
| Tumorigenic | Yes, in nude mice; forms colonies in agarose. |
| Receptor Expression | complement, expressed; Fc, expressed |
| Biosafety Level | BSL-2 |
Documents
MSDSPublications
Journal: Nature Communications (2026) IF: 15.7
DOI: 10.1038/s41467-025-67710-8
Product Cited: Daudi Cells Line
Journal: Current Research in Microbial Sciences (2026) IF: 5.8
DOI: 10.1016/j.crmicr.2026.100572
Product Cited: Ramos Cells Line, C666-1 Cells Line, Jurkat, Clone E6-1 Cells Line, AGS Cells Line, Akata Cells Line, CNE-2Z Cells Line, B95-8 Cells Line, 293T Cells Line, HNE-1 Cells Line, Raji Cells Line, Daudi Cells Line
Journal: Journal for ImmunoTherapy of Cancer (2025) IF: 10.6
Product Cited: MDA-MB-436 Cells Line, MDA-MB-231 Cells Line, MDA-MB-468 Cells Line, Daudi Cells Line, HCC1937 Cells Line, BT-549 Cells Line, Hs 578T Cells Line, HCC1806 Cells Line
Journal: Frontiers in Immunology (2024) IF: 5.7
DOI: 10.3389/fimmu.2024.1400177
Product Cited: HCT 116 Cells Line, Raji Cells Line, SGC-7901 Cells Line, Human Peripheral Blood Monocyte Cells, Daudi Cells Line, MKN-45 Cells Line, K-562 [K562] Cells Line, SK-OV-3 Cells Line, Jurkat76 Cells Line, MDA-MB-231 Cells Line, MCF 10A Cells Line, SK-BR-3 [SKBR3] Cells Line, 293T [HEK-293T] Cells Line
Journal: PLoS One (2023) IF: 3.7
DOI: 10.1371/journal.pone.0294729
Product Cited: Daudi Cells Line, Ramos [RA 1] Cells Line, DoHH2 Cells Line, Farage Cells Line, Raji Cells Line
