DLD-1

Name: DLD-1
Brand: Procell
SKU: CL-0074
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0074

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CL-0074

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	DLD-1
Synonyms	DLD 1;DLD1;CoCL3
Cellosaurus Accession	CVCL_0248
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	colorectal cancer cells
Tissue	Large intestine; Colon
Age	Adult
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:8
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The DLD-1 cell line is one of two colorectal adenocarcinoma cell lines isolated by D.L. Dexter and his colleagues between 1977 and 1979. DNA fingerprinting and karyotype analysis performed at ATCC and elsewhere have shown that DLD-1 cells are similar to HCT-15 cells, indicating that these two cell lines are different clones derived from the same individual. The genetic origin of DLD-1 cells can be confirmed by DNA fingerprinting, but karyotype analysis reveals that they lack consistent alterations in chromosomal markers or numbers. DLD-1 cells are CSAp negative (CSAp⁻). They exhibit positive p53 antigen expression, resulting from a C→T point mutation that leads to a Ser→Phe substitution at position 241 (c.722C>T, p. Ser241Phe). DLD-1 cells are positive for keratin by immunoperoxidase staining. They show positive expression of the oncogenes c-myc, K-ras, H-ras, N-ras, myb, sis, and fos; expression of the N-myc oncogene was not tested. DLD-1 cells express the tumor-specific nuclear matrix proteins CC-2, CC-3, CC-4, CC-5, and CC-6. The culture submitted to ATCC in 1979 was of unknown passage number and was contaminated with mycoplasma. Subsequently, it was treated with a combination of multiple antibiotics for 12 weeks. After treatment, it was tested weekly using Hoechst staining and standard culture methods. The cells were then cultured without antibiotics for 11 consecutive months, and all tests for mycoplasma remained negative.
Tumorigenic	Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells).
Antigen Expression	Blood Type O. The cells are weakly positive for keratins and vimentin. The cells are positive for keratin by immunoperoxidase staining. DLD-1 cells are positive for p53 antigen expression (the p53 antigen produced has a C -&amp;amp;gt; T mutatio
Gene Expression	carcinoembryonic antigen (CEA) 0.5 ng/10⁶ cells/10 days; colon antigen 3.
Biosafety Level	BSL-1