DMEM (Glucose free), with L-alanyl-L-glutamine, HEPES, without phenol red

Name: DMEM (Glucose free), with L-alanyl-L-glutamine, HEPES, without phenol red
Brand: Procell
SKU: PM150291
Price: 35.0 USD
Availability: InStock

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Cat. No.: PM150291

Manual

Formulation

DMEM (Glucose free), with L-alanyl-L-glutamine, HEPES, without phenol red - 1

PM150291

$ 35.00

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500mL

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Product Introduction Related Products Documents FAQs

Product Introduction

Background

DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times.
The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture.
DMEM medium without glucose can be added to the concentration of glucose at will according to the research needs, which is convenient and quick.
DMEM (glucose free) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.

Ingredient Statement

With

• 25 mM HEPES

• 4 mM L-Alanyl-L-Glutamine

• 3700 mg/L NaHCO₃

• 1 mM Sodium Pyruvate

Without

• D-Glucose

• Phenol red

Matters Need Attention

1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's DMEM (Glucose free), with L-alanyl-L-glutamine, HEPES, without phenol red complies with ISO 13485, ISO 9001.

Specifications

Concentration	1×
Volume	500mL
Form	Liquid
Bacteria Detection	Negative
Fungi Detection	Negative
Mycoplasma Detection	Negative
Endotoxin Content	< 3 EU/mL
pH	7.2-7.4
Animal Origin Ingredient	Without
Green Features	Sustainable packaging
Valid Period	24 months
Storage Condition	This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition	Room Temperature

Related Products

DMEM (Glucose free)

Catalog Number: PM150270

DMEM (Glucose free), with HEPES

Catalog Number: PM150274

DMEM (Glucose free), with L-alanyl-L-glutamine

Catalog Number: PM150285

Documents

Manual

MSDS

Formulation

FAQs

Q:1 Why is DMEM high glucose (phenol red-free) medium pale yellow?
Answer
The pale yellow color is due to ferric nitrate in the formulation. Ferric ions (Fe³⁺) in aqueous solution impart a light yellow coloration even at low concentrations, resulting in the observed appearance of the medium.
Q:2 If the basic culture medium is frozen at -20℃, will it affect its use?
Answer
If the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
Q:3 How long can I keep my culture media after supplementing with serum?
Answer
Generally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
Q:4 My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
Answer
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Q:5 How light sensitive is media? Should I also be protecting it from LED light?
Answer
While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
Q:6 I see a precipitate in my medium, and a change in the pH. What should I do?
Answer
This is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
Q:7 Where can I find the osmolality for Medium?
Answer
The osmolality is listed in the COA for the particular lot number of the medium.
Q:8 Do you provide customized culture media services?
Answer
Yes. We can customize culture media with reduced components or changes in the concentration of existing components.