DMS 153
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Cat. No.: CL-0558
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CL-0558
$ 420.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | DMS 153 |
| Synonyms | DMS-153;DMS153;Darmouth Medical School 153 |
| Cellosaurus Accession | CVCL_1175 |
| Organism | Human |
| Growth Properties | Aggregated suspension with loose adherence |
| Morphology | Lymphoblast-like |
| Disease | lung cancer cells |
| Tissue | Lung |
| Age | 44Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This suspension cell line tends to adhere. For long-term culture and expansion, it is recommended to use non-TC-treated vessels to maintain long-term stable cell condition and proliferation rate. Cells grow in aggregates; excessively large cell aggregates will reduce the proliferation rate. When fewer than 4–5 cell aggregates are observed under 100× magnification, consider dispersing the aggregates before continuing culture. 4. Using TC-treated dishes or flasks will cause cells to adhere, resulting in slow growth, increased dead cells, and deteriorated cell condition; this is not recommended for long-term culture. |
| Complete Medium | Waymouth's MB 752/1 [PM151215]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 times per week |
| Subculturing Procedure | 1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Weakly adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. For firmly adherent cells, rinse with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 4. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The DMS 153 cell line was established in 1976 by O.S. Pettengill and G.D. Sorenson from liver tissue of a 44-year-old Caucasian male with small cell lung cancer. DMS 153 cells secrete calcitonin, human calcitonin gene-related peptide, and adrenocorticotropic hormone (ACTH). The cells are tumorigenic in nude mice. |
| Tumorigenic | Yes; Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10⁷ cells. |
| Receptor Expression | Bombesin; epidermal growth factor (EGF); complement (CR3) |
| Antigen Expression | Leu 7 +; My23 +; CD11b + |
| Gene Expression | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; calcitonin gene related peptide (CGRP); oxytocin - neurophysin (OT-NP); 17 beta estradiol,Leu 7+; My23+; CD11b+ |
| Biosafety Level | BSL-1 |
MSDS
