DMS 79
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Cat. No.: CL-1186
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CL-1186
$ 600.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | DMS 79 |
| Synonyms | DMS-79;DMS79;Darmouth Medical School 79 |
| Cellosaurus Accession | CVCL_1178 |
| Organism | Human |
| Tissue | Lung; derived from metastatic site: pleural effusion |
| Age | 65Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150 °C to −196 °C. Storage at −80 °C is restricted to short-term interim use only. |
Reference materials
| Background | DMS 79 is a cell line isolated from the pleural effusion of a 65-year-old white patient with carcinoma. |
| Tumorigenic | Yes; Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107cells. |
| Antigen Expression | Leu 7; My23 |
| Gene Expression | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; corticotropin; beta endorphin; 17 beta estradiol; lipotropin; oxytocin - neurophysin (OT-NP); parathormone; somatostatin-like immunoreactivity (SRIF) |
| Biosafety Level | BSL-1 |
MSDS
