DU4475

Name: DU4475
Brand: Procell
SKU: CL-0719
Price: 460.0 USD
Availability: InStock

Copy the product info.

Cat. No.: CL-0719

Publications(1)

Select a size

1×10^6Cells/Vial×2Vials

Quantity

- +

In stock

Inquire

For order requirements and technical needs, please Contact Us

Product Introduction Documents Publications

General information

Cell Name	DU4475
Synonyms	Du4475;DU-4475;Du-4475;DU 4475;Du 4475;Duke University 4475
Cellosaurus Accession	CVCL_1183
Organism	Human
Growth Properties	Suspension with multicellular aggregation
Morphology	Round; epithelial-like
Disease	breast cancer cells
Tissue	Mammary gland/breast; derived from metastatic site: skin
Age	62Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:3
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	90% FBS+10% DMSO
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	DU4475 cell line was established from a locally metastatic cutaneous nodule of an undifferentiated carcinoma in a 62-year-old woman. This nodule developed seven years after she underwent radical mastectomy for a poorly differentiated ductal carcinoma of the breast. Literature reports indicate that the DU4475 cells grow readily in athymic nude mice and are capable of forming tumors. Additionally, these cells have been shown to express components of milk fat globules and are triple-negative (estrogen receptor-negative, progesterone receptor-negative, and human epidermal growth factor receptor 2-negative).
Tumorigenic	Yes, in nude mice.
Biosafety Level	BSL-1