DYR0100
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Cat. No.: CL-1059
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CL-1059
$ 600.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | DYR0100 |
| Synonyms | iPSDYR0100;ATCC-DYR0100-A;CNNDi001-A;ATCC-DYR0100 |
| Cellosaurus Accession | CVCL_C253 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Spherical colonies |
| Tissue | Skin; Foreskin |
| Age | <1M |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Abundant debris appears during culture of this cell line; timely medium exchange and washing are required. Perform cell recovery and culture only after all preparations are complete. 4. The working concentration of Y-27632 dihydrochloride solution is 10 μM. In stem cell culture, Y-27632 dihydrochloride solution is used during cryopreservation, recovery, and subculture; it is not required during medium exchange. 5. For culturing this cell line, please prepare the following in advance: Stem Cell Culture Coating Solution (1×) (Cat. No.:PB180537), Y-27632 Dihydrochloride Solution (Cat. No.:PB180609), and stem cell dissociation solutions. Options include: Accutase Cell Dissociation Solution (Cat. No.: PB180201), Recombinant Trypsin Dissociation Solution (with phenol red) (Cat. No.:PB180241), and Recombinant Trypsin Dissociation Solution (without phenol red) (Cat. No.:PB180240). |
| Complete Medium | Complete culture medium for DYR0100 [CM-1059] |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | Once daily |
| Dissociation Duration | 3-5 min |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | DYR0100 is a human induced pluripotent stem cell (iPSC) line derived from the foreskin fibroblast cell line SCRC-1041 via retrovirus-mediated reprogramming with the OCT4, SOX2, KLF4, and MYC genes. |
| Biosafety Level | BSL-2 |
MSDS
