EB-3
Copy the product info.
Cat. No.: CL-0334
-
-
-
- +2
CL-0334
$ 420.00
Select a size
1×10^6Cells/Vial×2Vials
Quantity
-
+
In stock
InquireFor order requirements and technical needs, please Contact Us
Product Introduction
General information
| Cell Name | EB-3 |
| Synonyms | EB-3;Epstein-Barr-3;GM04679 |
| Cellosaurus Accession | CVCL_1185 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | lymphoma cells |
| Tissue | Peripheral blood |
| Age | 3Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 3×10⁵-5×10⁵ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | EB-3 cells were derived from B lymphocytes obtained from a 3-year-old Black boy with Burkitt's lymphoma, and are EBNA positive. |
| Antigen Expression | HLA A3, Aw32, Cw2 |
| Biosafety Level | BSL-2 [Cells Contain HERPESVIRUS] |
MSDS
