ES-2

Name: ES-2
Brand: Procell
SKU: CL-0079
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0079

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CL-0079

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	ES-2
Synonyms	ES2
Cellosaurus Accession	CVCL_3509
Organism	Human
Growth Properties	Adherent
Morphology	Fibroblast-like
Disease	ovarian cancer cells
Tissue	Ovary
Age	47Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Upon entering the logarithmic growth phase, the cells proliferate rapidly and reach full confluency within 2–3 days when subcultured at a 1:2 split ratio. The medium is prone to rapid acidification, accompanied by the formation of cell aggregates. Partial cell detachment may occur after cryovial filling.
Complete Medium	McCoy's 5A [PM150710]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The ES-2 cell line was established from a surgical tumor specimen obtained from a 47-year-old Black female patient with poorly differentiated ovarian clear cell carcinoma. ES-2 cells were initially isolated by growth in soft agar and are tumorigenic in nude mice. The cells exhibit low to moderate resistance to various chemotherapeutic agents, including doxorubicin, cisplatin, carmustine, and etoposide, and express low levels of P-glycoprotein.
Tumorigenic	Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells.
Gene Expression	P glycoprotein
Biosafety Level	BSL-1