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Product Introduction
| Cell Name | FaDu |
| Synonyms | FaDU;FADU |
| Cellosaurus Accession | CVCL_1218 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Tissue | Pharynx; hypopharynx |
| Age | 56Y |
| Sex | Male |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:6 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 4-5 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | The FaDu cell line was established in 1968 from a punch biopsy of a hypopharyngeal tumor in a patient of Indian descent. FaDu cells exhibit bundled filaments in the cytoplasm and prominent desmosomes at cell–cell boundaries. |
Documents
MSDSPublications
Journal: Oncogenesis (2024) IF: 5.9
DOI: 10.1038/s41389-024-00536-z
Product Cited: FaDu Cells Line, Detroit 562 Cells Line
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024) IF: 5.6
DOI: 10.3390/ijms25115961
Product Cited: Detroit 562 Cells Line, FaDu Cells Line, HBE Cells Line
Journal: Aging-US (2024) IF: 3.9
Product Cited: FaDu Cells Line, MEM, with NEAA Medium
Journal: Heliyon (2024) IF: 3.4
DOI: 10.1016/j.heliyon.2024.e32522
Product Cited: FaDu Cells Line, CAL 27 Cells Line
Journal: Heliyon (2024) IF: 3.4
DOI: 10.1016/j.heliyon.2024.e32969
Product Cited: OECM-1 Cells Line, Human Oral Mucosal Fibroblast Cells, HSC-3 Cells Line, FaDu Cells Line, SCC-25 [SCC 25; SCC25] Cells Line, HN6 Cells Line
