GM23255
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Cat. No.: CL-1119
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CL-1119
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | GM23255 |
| Cellosaurus Accession | CVCL_5S83 |
| Organism | Human |
| Growth Properties | Suspension with clustering |
| Morphology | Lymphoblast-like; suspension growth with cell clumps |
| Tissue | Peripheral blood |
| Age | 16Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+15% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | GM23255 is an immortalized cell line obtained by immortalizing B lymphocytes with Epstein-Barr virus (EBV). It was derived from a 16-year-old Asian male patient with spinal muscular atrophy (SMA). The donor has 0 copies of the SMN1 gene and 3 copies of the SMN2 gene. |
| Biosafety Level | BSL-2 |
MSDS
