Grace's supplement medium for insect Cells
Manual
PM152012
$ 58.00
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Product Introduction
Background
Grace's supplement medium for insect cells was originally designed for cell growth of Antherea eucalypti, and is widely used for the cell growth of Spodoptera frugiperda, such as Sf9 and Sf21 cells. Sf9 and Sf21 cells are commonly used in baculovirus expression vector system (BEVS) to express recombinant proteins.
This product contains hydrolyzed milk protein, yeast powder, L-glutamine and sodium bicarbonate. In addition, this product needs to be used with serum or serum-free additives.
Ingredient Statement
With
• 700 mg/L D-Glucose
• 600 mg/L L-Glutamine
• 350 mg/L NaHCO₃
• 400 mg/L D (-)-Fructose
• 26680 mg/L D (+)-Sucrose
• 3303 mg/L Hydrolysed Protein
• 3330 mg/L Yeast
Without
• HEPES
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Grace's supplement medium for insect Cells complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 6.1-6.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 12 months |
| Storage Condition | This product can be stored at 2-8℃ for 12 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Documents
FAQs
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Q:1 What culture media are suitable for insect cell growth?
AnswerMedia suitable for insect cell culture include Grace’s Insect Medium (PM152011), TNM-FH Medium (PM152010), Grace’s Supplemented Insect Medium (PM152012), the SF-900 series serum-free media, Express Five SFM, and BaculoGold medium. These media are specifically formulated for lepidopteran insect cell lines, such as Sf9 and Sf21 cells derived from the fall armyworm (Spodoptera frugiperda), and High Five (BTI-TN-5B1-4) cells derived from the cabbage looper (Trichoplusia ni). They differ significantly from mammalian cell culture media, such as DMEM or RPMI 1640, in terms of nutrient composition, pH buffering systems, and osmolarity.
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Q:2 If the basic culture medium is frozen at -20℃, will it affect its use?
AnswerIf the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
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Q:3 How long can I keep my culture media after supplementing with serum?
AnswerGenerally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
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Q:4 My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
AnswerWe routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
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Q:5 How light sensitive is media? Should I also be protecting it from LED light?
AnswerWhile we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
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Q:6 I see a precipitate in my medium, and a change in the pH. What should I do?
AnswerThis is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
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Q:7 Where can I find the osmolality for Medium?
AnswerThe osmolality is listed in the COA for the particular lot number of the medium.
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Q:8 Do you provide customized culture media services?
AnswerYes. We can customize culture media with reduced components or changes in the concentration of existing components.
