Granta-519
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Cat. No.: CL-1038
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CL-1038
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | Granta-519 |
| Synonyms | GRANTA-519;Granta 519;Granta519;GRANTA519;G-519;G519 |
| Cellosaurus Accession | CVCL_1818 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Round and oval cells, growing singly or in small suspension clusters |
| Tissue | Peripheral blood |
| Age | 58Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. A portion of non-viable cells are present immediately post-thaw; these can be removed via low-speed centrifugation in subsequent cultivation steps. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 5×10⁵-1×10⁶cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | Granta-519 was established from the peripheral blood taken in 1991 at relapse of a high-grade B-NHL (leukemic transformation of mantle cell lymphoma, stage IV) diagnosed in a 58-year-old woman with previous history of cervical carcinoma. |
| Biosafety Level | BSL-1 |
MSDS
