H9 [derivative of HuT 78]

Name: H9 [derivative of HuT 78]
Brand: Procell
SKU: CL-0499
Price: 460.0 USD
Availability: InStock

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Cat. No.: CL-0499

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1×10^6Cells/Vial×2Vials

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Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	5×10⁵-2×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	H9 [derivative of HuT 78] cells are a clonal derivative of HuT 78 cells, express the CD3 and CD4 surface markers. Studies have shown that H9 cells are susceptible to human immunodeficiency virus type 1 (HIV-1) and can be used for the detection, isolation, and propagation of HIV-1, as well as for studies of other human T-cell lymphotropic viruses.
Biosafety Level	BSL-1