HCC 94 [HCC941122]

Name: HCC 94 [HCC941122]
Brand: Procell
SKU: CL-0277
Price: 360.0 USD
Availability: InStock

Copy the product info.

Cat. No.: CL-0277

Publications(3)

CL-0277

$ 360.00

Select a size

1×10^6Cells/Vial×2Vials

Quantity

- +

In stock

Inquire

For order requirements and technical needs, please Contact Us

Product Introduction Documents Publications

Product Introduction

General information

Cell Name	HCC 94 [HCC941122]
Synonyms	HCC-94;HCC94
Cellosaurus Accession	CVCL_6945
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	cervical cancer cells
Tissue	Uterus; Cervix
Age	37Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

HCC 94 [HCC941122] cells were derived from a highly differentiated uterine squamous cell carcinoma. The cell line was established in 1995 from the second-passage tumor tissue of a nude mouse xenograft (HCC 94V), which was derived from a human cervical well-differentiated squamous cell carcinoma. After adherent culture, cell growth initiated on day 6, and the first passage was performed on day 38. HCC 94 [HCC941122] cells can form tumors when subcutaneously transplanted into BALB/c-nu/nu nude mice.