HCC1937

Name: HCC1937
Brand: Procell
SKU: CL-0093
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0093

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CL-0093

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	HCC1937
Synonyms	HCC-1937
Cellosaurus Accession	CVCL_0290
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	breast cancer cells
Tissue	Breast; Duct; Mammary gland
Age	23Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The HCC1937 cell line was initially derived from a primary ductal carcinoma on October 13, 1995, and took 11.5 months to establish. The tumor was classified as TNM stage IIB, grade 3. BRCA1 analysis revealed that HCC1937 cells are homozygous for the BRCA1 5382C mutation, whereas a lymphoblastoid cell line derived from the same patient is heterozygous for this mutation. Two other family members also carry this mutation; an identical twin sister also suffered from breast cancer. HCC1937 cells harbor an acquired TP53 mutation with loss of the wild-type allele, an acquired homozygous deletion of the PTEN gene, as well as loss of heterozygosity at multiple loci known to be involved in the pathogenesis of breast cancer. HCC1937 cells are negative for both HER2 and p53 expression. The epithelial cell-specific markers epithelial glycoprotein 2 (EGP2) and cytokeratin 19 are both positive in HCC1937 cells; estrogen receptor (ER) and progesterone receptor (PR) expression are negative.
Receptor Expression	estrogen receptor, negative; progesterone receptor, negative
Gene Expression	Epithelial glycoprotein 2 (EGP2); cytokeratin 19
Biosafety Level	BSL-1