HCC2935
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Cat. No.: CL-1113
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CL-1113
$ 600.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | HCC2935 |
| Cellosaurus Accession | CVCL_1265 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Tissue | Lung; derived from metastatic site: pleural effusion |
| Age | 39Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. More dark granules are present upon cell recovery and persist throughout the culture process, along with some floating cells. Treat with medium exchange. 4. This cell line proliferates slowly, with a doubling time of approximately 5–7 days. The recovery period is relatively long. If proliferation ceases midway, dissociate and re-plate the cells. 5. Subculture is recommended when cell density reaches 80% or above. If the initial seeding density is low, subsequent subculture density will be low and cell growth will be extremely slow. Maintain a subculture split ratio of 1:2, not exceeding 1:3. 6. Due to slow proliferation, seeding in 6-well plates is recommended for cell recovery, followed by culture in low-density vessels. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 4-5 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | HCC2935 is an epithelial cell line that was isolated in 1999 from the pleural effusion of a White, 39-year-old, male patient with adenocarcinoma. |
| Biosafety Level | BSL-1 |
MSDS
