HCC827-ADR
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Cat. No.: CL-1155
CL-1155
$ 720.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | HCC827-ADR |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | lung cancer cells |
| Tissue | Lung |
| Age | 39Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. With a relative resistance index greater than 6, ADR supplementation is not required during recovery. Culture cells in standard RPMI-1640 complete growth medium. Subsequently, adjust the ADR concentration based on cell condition. The recommended range is 0.25–0.5 μg/mL. Further increasing the ADR concentration may result in slower cell proliferation and increased cell lysis. Culturing without ADR for more than two weeks will significantly reduce the drug resistance index. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+0.5 μg/mL Supplement1+1% Supplement2 |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The HCC827 cell line was established in March 1994. The tissue donor smoked one pack of cigarettes per month at 25–26 years of age and quit smoking 12 years prior to diagnosis. HCC827 cells harbor a somatic deletion (E746–A750 deletion) in the tyrosine kinase domain of EGFR. |
| Biosafety Level | BSL-1 |
MSDS
