HCCLM3

Name: HCCLM3
Brand: Procell
SKU: CL-0278
Price: 360.0 USD
Availability: InStock

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Cat. No.: CL-0278

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CL-0278

$ 360.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	HCCLM3
Synonyms	LM3;MHCC-LM3;MHCCLM3
Cellosaurus Accession	CVCL_6832
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	intrahepatic cancer cells
Tissue	Liver
Age	39Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	DMEM [PM150210]+20% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2
Medium Renewal	Every 2 to 3 days
Dissociation Duration	2-4 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

HCCLM3 cells are a hepatocellular carcinoma cell line with high spontaneous pulmonary metastatic potential. This cell line was established by inoculating nude mice with the human hepatocellular carcinoma cell line MHCC97-H, followed by three rounds of in vivo selection for pulmonary metastasis. It was derived from harvested lung metastatic nodules and subsequently passaged subcutaneously. HCCLM3 cells are widely used in basic and clinical research on the pathogenesis of human hepatocellular carcinoma, as well as in the screening of antitumor drugs.

Biosafety Level

BSL-1