Hce-8693
Copy the product info.
Cat. No.: CL-0348
-
-
-
- +2
CL-0348
$ 360.00
Select a size
1×10^6Cells/Vial×2Vials
Quantity
-
+
In stock
InquireFor order requirements and technical needs, please Contact Us
Product Introduction
General information
| Cell Name | Hce-8693 |
| Synonyms | Hce-8693;HCE-8693;Hce8693;HCE8693 |
| Cellosaurus Accession | CVCL_6909 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | colorectal cancer cells |
| Tissue | Cecum; derived from metastatic site: lymph node |
| Age | 53Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | Hce-8693 cells were derived from a pathological section of lymph node metastasis from a male patient with undifferentiated adenocarcinoma of the cecum. The doubling time of Hce-8693 cells is 30.4 hours, with a mitotic index of 28.8%. Electron microscopy reveals that Hce-8693 cells exhibit large nuclei with distinct nucleoli, abundant microfilaments, and secretory granules. The modal chromosome number is 48, and the colony-forming efficiency in soft agar is 8%. Culture supernatants of Hce-8693 cells test positive for CEA. Following heterotransplantation into nude mice, tumors formed by Hce-8693 cells resemble the original patient lesion, with Alcian blue-positive material present in the cytoplasm. |
MSDS
