HCT 116 (3D)
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Cat. No.: CL-0096-3D
CL-0096-3D
$ 720.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | HCT 116 (3D) |
| Organism | Human |
| Growth Properties | Suspension spheres |
| Morphology | Tight 3D spheres |
| Disease | colorectal cancer cells |
| Tissue | Colon |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. When cultured in non-low-attachment vessels, tumor spheres may exhibit partial adherent growth. |
| Complete Medium | Complete culture medium for HCT 116 (3D) |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 0.5×10⁵ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 3-5 min |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | HCT 116 tumorspheres are cancer stem cell-enriched spheroids obtained from HCT 116 cells by enrichment and culture with specialized medium. |
| Biosafety Level | BSL-1 |
MSDS
