HCT-15-5FU
Copy the product info.
Cat. No.: CL-1011
-
-
-
- +2
CL-1011
$ 720.00
Select a size
1×10^6Cells/Vial×2Vials
Quantity
-
+
In stock
InquireFor order requirements and technical needs, please Contact Us
Product Introduction
General information
| Cell Name | HCT-15-5FU |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | colorectal cancer cells |
| Tissue | Large intestine; Colon |
| Age | 67Y |
| Sex | 男性 |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. For initial culture, when cell confluence reaches approximately 80%, add complete medium containing 10–16 μg/mL 5-FU and culture until cells reach full confluence, then subculture. After 1–2 subcultures, if cells still maintain proliferation, the drug concentration can be increased to 20 μg/mL for continued culture. If cells stop proliferating and exhibit poor condition during this process, the drug concentration needs to be reduced (halve the drug concentration for the first reduction) or culture in drug-free complete medium. Wait until cell confluence reaches approximately 80% and cells are in good growth condition, then switch to the required 5-FU drug concentration. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+20 μg/mL Supplement1+1% Supplement2 |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:8 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 3-5 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | HCT-15-FU is a 5-FU-resistant subline established from HCT-15 cells. |
| Biosafety Level | BSL-2 |
MSDS
