HEC-1-B

Name: HEC-1-B
Brand: Procell
SKU: CL-0100
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0100

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CL-0100

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	HEC-1-B
Synonyms	Hec-1-B;HEC-1B;HEC1-B;HEC1B;Hec1B
Cellosaurus Accession	CVCL_0294
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Uterus; Endometrium
Age	71Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	HEC-1-B cells are a substrain of HEC-1-A cells, isolated by H. Kuramoto in 1968. In contrast to HEC-1-A cells, HEC-1-B cells exhibit a stationary growth phase between days 135 and 190 in culture. Upon recovery, they appear flattened and display a more pavement-like morphology relative to the parental cell line. Furthermore, the modal chromosome complement of HEC-1-B cells is double that of the parental cell line.
Tumorigenic	Yes, in nude mice (The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma (grade II)). Yes, in the cheek pouch of cortisone treated hamsters (The cells form typical papillary adenomas).
Antigen Expression	Blood Type B; Rh+
Biosafety Level	BSL-1